解:
$\because$ 实数$s,t$满足$2s^2+3s-1=0,$$2t^2+3t-1=0,$且$s≠ t,$
$\therefore s,t$是一元二次方程$2x^2+3x-1=0$的两个不相等的实数根,
由韦达定理得:$s + t = -\frac{3}{2},$$st = -\frac{1}{2}。$
$\because (t-s)^2=(t+s)^2-4st=(-\frac{3}{2})^2-4×(-\frac{1}{2})=\frac{17}{4},$
$\therefore t-s=\pm\frac{\sqrt{17}}{2}。$
$\therefore \frac{1}{s}-\frac{1}{t}=\frac{t-s}{st}=\frac{\pm\frac{\sqrt{17}}{2}}{-\frac{1}{2}}=\pm\sqrt{17}。$